Manik, Ramona Yulianti (2023) Pengaruh Naa dan Bap Terhadap Pertumbuhan Tanaman Anggrek Dendrobium Sp) Secara In-vitro. Other thesis, Universitas Islam Riau.

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Abstract

The aim of this study was to determine the interaction effect as well as the main effect of NAA and BAP on the growth of orchid plants in vitro. The research was carried out at the Faperta-UIR Biotechnology Laboratory Jalan Kharudin No. 113, Bukit Raya District, Air cold Village, Pekanbaru, starting from December 2022 to March 2023. Using the RAL Factorial, the first factor is the concentration of NAA (0; 0.5; 1 and 1.5 ppm). The second factor is the concentration of BAP (0, 2, 4, and 6 ppm). In order to obtain 16 treatment combinations with 3 replications, there were 48 experimental units. One experimental unit consisted of 3 bottles each containing 4 plants and 2 bottles were used as samples, so that the total bottles were 144 bottles. Parameters observed were the percentage of live explants, the percentage of explants that formed shoots, the percentage of explants that formed roots, the age at which shoots appeared, the number of shoots per explant, the number of roots per explant, and the number of candidate plantlets per explant. Observational data were analyzed statistically and continued with the BNJ test at the 5% level. The results showed that the interaction between NAA and BAP in orchid plant explants with NAA concentration of 1.5 ppm and BAP 2 ppm was significantly different in the parameters of the percentage of explants forming roots, the age at which shoots appeared, the number of shoots per explant, the number of roots per explant, and the number of candidates. plantlets per explant. The main effect of NAA was significantly different for all observed parameters of NAA concentration of 1.5 ppm. The main effect of BAP was significantly different for all parameters except for the percentage of live explants, the best BAP was given at a concentration of 2 ppm.

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