Oktavianti, Ria (2018) Aplikasi PCR (Polimerase Chain Reaction) Menggunakan Primer Spesifik Untuk Mendeteksi Cabai yang Toleran Terhadap Kekeringan. Other thesis, Universitas Islam Riau.

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Abstract

Ria oktavianti (144120008), PCR (Polymerase Chain Reaction) application using specific primers to detect drought tolerant ekillies. Supervised by Ir. Saripah Ulpah, M. Sc, Ph. D (Main Supervisor), Dr. Maizar, M.P (CoSupervisor) and Dr. Rosmaina M.Si (Technical Supervisor). This research was carried out in the Laboratory of Plant Breeding and Genetics of UIN SUSKA Riau, and the implementation of PCR in the Genetic Laboratory in the University of Riau. This research was done for 2 months, starting from May 2018 to June 2018. The purpose of this study was to find tolerant genotypes. This research was conducted on 16 chilli genotypes from UIN Suska Riau Laboratory of Breeding and Genetics. Using four specific primers wich were TIL, DREB2A, P5CS, and Primary Pair 2 (BLAS). Results of the study showed amplifikasi of chilli DNA could be obtained using TIL and Pair 2. Primer however th corsponding result were obtain only by using TI to be drought tolerant, while 5 genotypes were found drought intolerant. The drought tolerant genotypes were (RFC-005), (RFC-006), (G-UIN- 016), (G-UIN-015), (G-UIN065), (UIN-097), (UIN-035), (UIN-100), (UIN-099), (UIN-037) and (UIN-096). While 5 intolerant genotypes were (G-UIN-019), (G-UIN-008), (G-UIN-038), (RFC-013) and (UIN-036).

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