Arnilan, Widya (2023) Pengaruh Konsentrasi dan Beberapa Jenis Auksin terhadap Induksi Perakaran Tanaman Anggrek (Dendrobium Sp) Secara In-vitro. Other thesis, Universitas Islam Riau.

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Abstract

The purpose of this study was to determine the effect of the interaction of concentrations and several types of auxin on the in-vitro induction of orchid plant roots. This research was carried out at the Biotechnology Laboratory of the Faculty of Agriculture, Islamic University of Riau, Jalan Kaharudin Nasution KM 11 No. 113 Air Cold Village, Bukit Raya District, Pekanbaru City. This research was conducted for 3 months starting from December 2022 to February 2023. This study used a split plot design in the form of a completely randomized design consisting of the main plot, namely the type of auxin with 3 treatments and the subplots, namely the concentration consisting of (0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm), so that there 12 treatment combinations with 3 replications. Thus the study consisted of 36 experimental units, each experimental unit contained 2 bottles, both of which were sampled, each bottle was planted with 2 plants so that a total of 144 plants. Parameters observed were: percentage of living explants, age at which roots appeared, number of roots per explant, longest root length and percentage of explants forming roots. The research data were analyzed statistically and continued with the 5% BNJ test. The results showed that the interaction effect of concentration and several types of auxin significantly affected the age of root emergence and the longest root length of Dendrobium sp. The main effect of several types of auxin significantly on the number of roots and the percentage of forming roots of Dendrobium sp orchid plants, the type of auxin is NAA. It can be concluded that the main effect of the concentration of several types of auxin is significant on the age of emergence of roots, the number of roots, the length of the longest roots and the percentage of forming roots, at the best concentration of 0.5 ppm.

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