Qadarsih, Desy (2025) Pengaruh Jenis dan Konsentrasi Nutrisi Terhadap Kandungan Protein Serta Produksi Mikroalga Chlorella Sp. Masters thesis, Universitas Islam Riau.

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Abstract

The research titled “The Influence of Nutrient Types and Concentrations on Protein Content and Production of Microalga Chlorella sp.” was conducted at the Algae Laboratory and Fisheries Chemistry Department, Faculty of Fisheries and Marine Sciences, Riau University. The study spanned a period of 4 months, from March 2024 to October 2024. The research involved several stages, including the fermentation of mustard vegetable waste, Padang restaurant waste, and fish waste, inoculation of microalgae, water quality measurements, biomass production trials, and protein content analysis of Chlorella sp. The parameters measured during the study included cell abundance, biomass production, nitrate and phosphate content, and protein content in each experimental unit. The study employed an experimental method with a 2-factor Randomized Complete Block Design (RCBD) for the first phase, with experimental units at concentrations of 25, 50, 75, and 100 ml for each waste type (mustard, restaurant, and fish), and concentrations of 10, 20, and 30 ml. In the second phase of the study, a single-factor design was used with 9 experimental units, each consisting of 10 ml with 3 repetitions. Phase I included three levels of treatment, three replications, and two control groups: a positive control (Dahril fertilizer) and a negative control (without fertilizer). The results indicated that the P1 treatment (mustard waste) produced the highest biomass and protein content, with values of 2.03 g/L and 22.17%, respectively. In contrast, the P3 treatment (fish waste) resulted in the lowest biomass (0.53 g/L) and protein content (4.27%). The P2 treatment (Padang restaurant waste) produced intermediate results, with a biomass of 0.97 g/L and protein content of 8.80%. These findings highlight the potential of vegetable waste as an effective fertilizer for enhancing Chlorella sp. production.

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