Antibacterial potential of bacteria isolates from Hypostomus plecostomus fish intestine against fish pathogens

Setiaji, Jarod and Iskandar Johan, Teuku and Heriyanto, Heriyanto and Marina Kuswari, Putri and Syaputra, Try (2023) Antibacterial potential of bacteria isolates from Hypostomus plecostomus fish intestine against fish pathogens. AACL Bioflux, 16 (4). pp. 2289-2298.

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Abstract

Diseases caused by bacteria have become are a serious problem in fish ecosystems leading to a significant number of deaths. Bacteria that often infect the fish are Aeromonas hydrophila, Aeromonas salmonicida, Edwardsiella ictaluri, Pseudomonas aeruginosa, and Vibrio alginolyticus. Antibiotics are generally used to cure bacterial diseases; however uncontrolled use can lead to bacteria developing resistance. One way to combat the resistance is by looking for new compounds from microbes. The objective of this research is to identify the type of bacterium from Hypostomus plecostomus intestine that can inhibit the growth of pathogen bacteria such as Aeromonas hydrophila, Aeromonas salmonicida, Edwardsiella ictaluri, Pseudomonas aeruginosa, and Vibrio alginolyticus. This research used an explorative method and experiment. Bacteria were isolated from the intestine of Hypostomus plecostomus fish. It was cultured on tryptic soy agar (TSA) medium and purified. The isolates from the purified breed went through antagonistic test by diffusion method against Aeromonas hydrophila, Aeromonas salmonicida, Edwardsiella ictaluri, Pseudomonas aeruginosa and Vibrio alginolyticus pathogen bacteria. The bacterium isolates from the fish intestine were identified by using the 16S rDNA. The antagonistic test results from the Hypostomus plecostomus fish intestine with the code JS1-22, JS2-22, JS3-22 that can inhibit pathogen bacteria growth in fish were categorized as very effective. The findings from using BLAST system on isolate JS1-22 isolate showed DNA similarity with Bacillus paramycoides (99.59%), JS2-22 isolate with Bacillus sp. (99.53%), and JS3-22 isolate with Bacillus megaterium (99.65%).

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