Activity of an antibacterial compound produced by Bacillus sp. strain JS4 (MT102913.1)

Setiaji, Jarod and Feliatra, Feli and Yuda Teruna, Hilwan and Lukistyowati, Iesje and Heriyanto, Heriyanto (2023) Activity of an antibacterial compound produced by Bacillus sp. strain JS4 (MT102913.1). AACL Bioflux, 16 (1). pp. 591-596.

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Abstract

Pathogenic bacteria are types of bacteria that often cause negative effects on fish and other organisms. Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio alginolyticus bacteria often infect farmed fish, causing death. The use of antibiotics to treat bacterial diseases can result in antibiotic residues in aquatic products and bacterial resistance. The heterotrophic microscopic organisms separates were gathered from ocean waters in Sungai Pakning Bengkalis Rule Riau Territory Indonesia. The execution season of this study was directed in May-November 2020. An antibacterial compound from Bacillus sp. strain JS4 can be used as an alternative to solve this problem. 12 liters of Bacillus sp. strain JS4 bacterium was cultured for 7 days on NB media, then extracted with ethyl acetate. The concentrate was disconnected utilizing streak chromatography. The concentrate was identified utilizing FT-IR and the compound was portrayed utilizing 1H NMR (Nuclear Magnetic Resonance) at 500 MHz recurrence and 13C NMR at 125 MHz. The compound test was finished by utilizing the agar dissemination strategy. The consequence of range examination NMR and Comfortable confirmed that compound JS4-E-01 was N-[2(3-hydroxypropanoyl)ethenyl]3-hydroxypropanamide. The restraint ability test JS4-E-01 on A. hydrophila (10.5 mm), P. aeruginosa (12.3 mm),and V. alginolyticus (13,3 mm). All in all, Bacillus sp. strain JS04 bacterium can deliver N-[2-(3-hydroxypropanoyl)ethenyl]3-hydroxypropanamide compound. This compound had the option to restrain the development of A. hydrophila, P. aeruginosa and V. alginolyticus microbe microscopic organisms.

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