In vitro flowering of Fortunella hindsii (Champ.)

Jumin, Hasan Basri and Nito, Nobumasa (1996) In vitro flowering of Fortunella hindsii (Champ.). Spinger. pp. 484-488.

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Abstract

Abstract. Branch internodes of mature plants and stem internodes of seedlings of Fortunella hindsii flowered in vitro on half-strength MT (Murashige and Tucker 1969) basal medium supplemented with benzyladenine, adenine, 6-~-~dimethylallylaminopurine and kinetin. The highest percentage of flowering was achieved with explants ori- ginating from branch internodes of flowering plants close to the apex on half-strength MT basal medium containing 5% sucrose and 0.01 mg 1-1 BA in light. Exposure to darkness for more than 3 weeks followed by re-exposure to light reduced flowering. Flowering required a 4-day exposure to BA, but shoot formation could be initiated even without exposure to BA. First branch internode segments on MT basal medium containing 5% sucrose were prolific in flower (85%) production. The sucrose treatment affected the flower bud size distribution. There were about 13 flower buds per culture in the largest size category (>5 ram).

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