Embryogenic Protoplast Cultures of Orange Jessamine (Murraya Paniculata) and Their Regeneration Into Plants Flowering in Vitro

Jumin, Hasan Basri and Nito, Nobumasa (1995) Embryogenic Protoplast Cultures of Orange Jessamine (Murraya Paniculata) and Their Regeneration Into Plants Flowering in Vitro. Spinger, 41. pp. 277-279.

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Abstract

Embryogenic callus was induced from the hypocotyl region of seedlings germinated from immature embryos of orange jessamine (Murraya paniculata (L.) Jack) on Murashige & Tucker (1969) medium containing 50 g 1-1 sucrose, 5.0 mg 1- l benzyladenine, 2.5 mg 1-1 2,4-dichlorophenoxyacetic acid and 600 mg 1-1 malt extract. Isolated protoplasts divided to produce callus on Murashige & Tucker (1969) medium containing 50 g 1-1 sucrose, 0.01 mg 1-1 gibberellin A4+7 and 600 mg 1-l malt extract. Callus developed to plantlets via somatic embryogenesis on Murashige & Tucker (1969) medium with 50 g 1 -l lactose but no plant growth regulators. These plantlets flowered in vitro on half strength Murashige & Tucker (1969) medium containing 50 g 1-l sucrose after 2 months culture.

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