Plant Regeneration Via somatic Embryogenesis from Protoplasts of Six Plant Species Related to Citrus

Jumin, Hasan Basri and Nito, Nobumasa (1996) Plant Regeneration Via somatic Embryogenesis from Protoplasts of Six Plant Species Related to Citrus. Spinger, 15. pp. 332-336.

Text Plant regeneration via somatic embryogenesis_six Plant.pdf - Published Version Download (866kB)

Abstract

Protoplasts isolated from embryogenic callus of Fortunella polyandra (Ridl.), Atalantia bilocularis (Pieree ex Guill.), Hesperethusa crenulata (Roxb.), Glycosmis pentaphylla (Re~.) Ca)rr., Triphasia trifolia (Burm. f.) P. Wils. and Murraya koenigii (L.) Spreng. were cultured in MT (Murashige and Tucker 1969) basal medium contain- ing 5% sucrose supplemented with 0.0, 0.001, 0.01, 0.1 or 1.0 mg 1-1 BA and 0.6 M sorbitol. The highest plating efficiencies for all species were obtained on MT basal medium containing 5% sucrose supplemented with 0.001 mg 1 -a B.A. E polyandra produced higher percentages of globular ,somatic embryo development, while A. bilocu- laris consistently showed a lower percentage of globular somatic embryo development in all 5 concentrations of BA. MT basal medium containing 5% sucrose and sup- plemented with 0.001 mg 1-1 BA was found to be a suit- able medium for development of globular somatic em- bryos derived from protoplasts to form heart-shaped somatic embryos with cotyledon-like structures. The highest percentages of shoot fl)rmation for all 6 species were obtained using 0.1 mg 1 -] GA. 3. A complete proto- plast-to-plant system was developed for E polyandra, A. bilocularis and Z trifolia, which could facilitate the trans- fer of nuclear and cytoplasmic genes from these species into cultivated Citrus through protoplast fusion.

Actions (login required)

View Item