Plant Regeneration Via Somatic Embryogenesis from Protoplast of Clausena Harmandiana (Engl.) Swing and M. Kell

Jumin, Hasan Basri (2013) Plant Regeneration Via Somatic Embryogenesis from Protoplast of Clausena Harmandiana (Engl.) Swing and M. Kell. Analele Universităţii din Oradea – Fascicula Biologie. pp. 23-28. ISSN 1224-5119

Text Plant Regeneration Via Somatic Embryogenesis.pdf - Published Version Download (652kB)

Abstract

Protoplasts isolated from embryogenic callus of Clausena harmandiana (Engl.) 'Swing. and M. Kell. were cultured in MT (Murashige and Tucker 1969) basal medium containing 5% sucrose supplemented with bezyladenine (BA), malt extract (ME) and 0.6 M sorbitol. The highest plating efficiency was obtained on MT basal medium containing 5% sucrose supplemented with 0.01 mg 1 ME, MT basal medium containing 5% sucrose and supplemented with 0.01 mg 1-1 BA and 600 mg 1-1-1 6(-y,y-dimethylallylamino)-purine was found to be a medium suitable for the development somatic embryos into heart-shaped somatic embryos. The highest percentage of shoot formation gibberellic acid (GA3) + 0.1 mg 1- was obtained using 0.1 mg 1-1-1 zeatin. In this investigation 25 plants were survived and grew normally in the soil.

Actions (login required)

View Item