Somatic Embryogenesis Via Protoplast Cultures of Fortunella Hindsii (Champ.) and Their Regeneration Into Precocious Flowering Plants

Jumin, Hasan Basri (2013) Somatic Embryogenesis Via Protoplast Cultures of Fortunella Hindsii (Champ.) and Their Regeneration Into Precocious Flowering Plants. Journal of Agricultural Technology, 9. pp. 999-1012. ISSN 1686-9141

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Abstract

Protoplasts isolated from embryogenic callus of Fortunella hindsii (Champ.) were cultured in MT (Murashige and Tucker, 1969) basal medium containing 5% sucrose supplemented with kinetin, malt extract (ME) and 0.6 M sorbitol. About 85% of the surviving protoplasts formed cell wall within 6 d of culture and the first cell division was observed 7 days after isolation. The highest plating efficiency was obtained in MT basal medium containing 5% sucrose supplemented with 0.01 mg 1 ME, MT basal medium containing 5% sucrose and supplemented with 0.01 mg 1-1-1 kinetin 600 mg 1 zeatin was found to be a medium suitable for the development somatic embryos into heart-shaped somatic embryos. The highest percentage shoot formation was obtained using 0.1 mg 1-1 -1 benzyladenine (BA) + 0.1 mg 1-1 gibberellic acid (GA3). In this investigation 40 plants survived and grew normally in the soil. After two months of being maintained in the soil, the plants formed flowers and the flowers developed into fruits in the soil treated with BA. Key words: cytokinin, Fortunella hindsii, flowering, protoplast, somatic-embryo, sucrose

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