Plant Regeneration Via Somatic Embryogenesis From Protoplasts Of Uganda Cherry Orange (Citropsis Schweinfurthii)

Jumin, Hasan Basri and Nito, Nobumasa (1996) Plant Regeneration Via Somatic Embryogenesis From Protoplasts Of Uganda Cherry Orange (Citropsis Schweinfurthii). Spinger, 15. pp. 754-757.

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Abstract

Protoplasts isolated from embryogenic callus of Citropsis schweinfurthii (Engl.) Swing. & M. Kell were cultured in MT (Murashige and Tucker 1969) basal medium containing 5% sucrose supplemented with 0.0, 0.001, 0.01, 0.1 or 1.0 mg 1-1 BA, 0, 300, 600 or 900 mg 1-1 malt extract and 0.6 M sorbitol. The highest plating efficiency was obtained on MT basal medium containing 5% sucrose supplemented with 0.01 mg 1-1 BA and 600 mg 1-1 malt extract. MT basal medium containing 5% sucrose and supplemented with 0.01 mg 1-1 kinetin was found to be a medium suitable for the development of globular somatic embryos derived from protoplasts into heart-shaped somatic embryos with cotyledon-like struc- tures. The highest percentage of shoot formation was obtained using 0.1 mg 1-1 GA 3. A complete protoplast- to-plant system was developed for C. schweinfurthii, which could facilitate the transfer of nuclear and cyto- plasmic genes from this species into cultivated Citrus through protoplast fusion.

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