Jumin, Hasan Basri and Nito, Nobumasa (1996) Plant Regeneration Via Somatic Embryogenesis From Protoplasts Of Uganda Cherry Orange (Citropsis Schweinfurthii). Spinger, 15. pp. 754-757.
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Abstract
Protoplasts isolated from embryogenic callus of Citropsis schweinfurthii (Engl.) Swing. & M. Kell were cultured in MT (Murashige and Tucker 1969) basal medium containing 5% sucrose supplemented with 0.0, 0.001, 0.01, 0.1 or 1.0 mg 1-1 BA, 0, 300, 600 or 900 mg 1-1 malt extract and 0.6 M sorbitol. The highest plating efficiency was obtained on MT basal medium containing 5% sucrose supplemented with 0.01 mg 1-1 BA and 600 mg 1-1 malt extract. MT basal medium containing 5% sucrose and supplemented with 0.01 mg 1-1 kinetin was found to be a medium suitable for the development of globular somatic embryos derived from protoplasts into heart-shaped somatic embryos with cotyledon-like struc- tures. The highest percentage of shoot formation was obtained using 0.1 mg 1-1 GA 3. A complete protoplast- to-plant system was developed for C. schweinfurthii, which could facilitate the transfer of nuclear and cyto- plasmic genes from this species into cultivated Citrus through protoplast fusion.
Item Type: | Article |
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Uncontrolled Keywords: | BA, N6-benzyladenine; 2,4-D, 2,4-dichlorophenoxyacetic acid; FDA, fluorescein diacetate; GAe, gibberellin A3; ME, malt extract |
Subjects: | S Agriculture > S Agriculture (General) |
Divisions: | > Agroteknologi |
Depositing User: | Mia |
Date Deposited: | 29 Aug 2022 07:49 |
Last Modified: | 29 Aug 2022 07:49 |
URI: | http://repository.uir.ac.id/id/eprint/14440 |
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